Next generation aptamers with unparalleled chemical diversity that significantly improves interaction with a target. X-Aptamers are selected using a non-enzymatic, bead-based process that enables combinations of chemical functionalities that are incompatible with traditional aptamer selection processes.
|X-Aptamers can include combinations of functionalities:||X-Aptamer benefits include:|
What is an X-Aptamer?
An X-Aptamer is an affinity agent (similar in function to an antibody) that is comprised of a single strand of DNA with one or more (usually several) non-DNA chemical modifications attached to one or more of the DNA bases.
How is an X-Aptamer different than an aptamer?
An X-Aptamer has much greater chemical diversity than an aptamer. X-Aptamers use combinations of chemically-modified DNA bases and backbones that typically cannot be amplified using PCR. This makes it impossible to select X-Aptamers using the typical enzymatic processes used to select aptamers (e.g. SELEX). The diversity of the chemical functional groups in X-Aptamers provides much better interaction with a target, which typically results in better binding affinity and specificity.
Why should I use an X-Aptamer instead of an antibody?
While antibodies are powerful and widely-used affinity agents, they have limitations that X-Aptamers overcome. X-Aptamers are ambient stable, have a long shelf life, can be developed in non-physiological conditions, and can be developed for targets that cannot be used to develop antibodies (toxins, targets that do not illicit an antibody response, etc.)
How does the X-Aptamer Selection Kit work?
The most important thing to know about the X-Aptamer Selection Kit is that it is not based on SELEX. AM Biotech has developed a two-step, bead-based method of quickly and easily identifying those sequences in the library that bind well with the target(s). The key to the X-Aptamer Selection Kit is the library of microbeads. Each microbead of the billions of microbeads in a library carries about 0.5 picomoles of just one unique, chemically-modified oligonucleotide. One or more of those chemically-modified oligonucleotides will be an X-Aptamer for a target and the kit protocol makes finding that bead/sequence relatively easy.
What do I need to perform a selection with a kit?
The X-Aptamer selection process uses simple tools and reagents typically found in most biochemistry laboratories. You will need access to pipettes, buffers, a simple magnetic stand, a thermocycler, gel electrophoresis equipment, and a centrifuge.
What kinds of targets are compatible with the kit?
Currently, the X-Aptamer Selection Kit is designed to work with proteins, structurally stable peptides, and small molecules.
How long does it take to complete the kit protocol?
A scientist can complete the protocol in two to four days depending upon his/her skill level and familiarity with some of the protocol steps.
What skills/training do I need to complete the kit protocol?
The kit protocol can be successfully completed by a scientist or lab technician who has some training and/or experience with basic biochemistry laboratory techniques. No special training is required.
How many X-Aptamers will I get from a kit?
Typically, for each target (up to 3) you used in the kit selection AM Biotech will send around four putative X-Aptamers. If you use the kit to select X-Aptamers to three targets simultaneously, then you will receive about 4 putative X-Aptamers per target (up to 12 total). If you use the kit to select X-Aptamers to just one target, you will receive up to 12 putative X-Aptamers to that single target. You will test these putative X-Aptamers for binding to your target(s). Some of the putative X-Aptamers that you receive may not bind to your target (false positives).
Will I get the sequences with the X-Aptamers?
AM Biotech provides commercial customers with the sequences and chemical modification pattern after a commercial license is purchased. A customer can use the X-Aptamers provided for unlimited research without a commercial license. Universities and other non-profit organizations can receive the sequence and modification information if required for acceptance of a publication in a scientific journal. However, if the university or non-profit does not submit a patent application for the X-Aptamer sequences, then AM Biotech will ask for performance data in return as well as the non-exclusive right to commercialize the X-Aptamers.
Can I get additional X-Aptamer material without a commercial license?
Yes. AM Biotech will synthesize additional X-Aptamer material for you at a reasonable price that would be comparable to what other oligo synthesis companies would charge for a similar oligonucleotide.
Please contact us if you have questions or would like additional information.
AM Biotechnologies, LLC
12521 Gulf Freeway, Houston, Texas 77034
Phone: (832) 295-1483
Email: info (at) am-biotech.com
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