Thiophosphoramidites are unique reagents that are used to replace the two non-bridging oxygen atoms with sulfur atoms in a DNA or RNA phosphodiester linkage creating a phosphorodithioate (PS2) linkage. AM sells these reagents through Glen Research Corporation.
• Like natural DNA (RNA), the phosphorodithioate linkage is achiral at phosphorus
• Eliminates stereoisomer formation associated with monothioate (PS) backbones
• PS2 analog is completely resistant to nuclease degradation
• PS2-ODNs bind proteins with a higher affinity than their phosphodiester analogues
• PS2 siRNA enhance gene silencing and increase RNA in vivo stability
Thiophosphoramidite Reagents (RNA or DNA)
• For use on automated DNA/RNA synthesizers to incorporate an achiral, dithio backbone (phosphorodithioate) at user selected positions.
• Coupling efficiency >97%
• High purity (~95% as measured by P31-NMR)
| DNA Thiophosphoramidites |
RNA Thiophosphoramidites |
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| Applications: |
Applications: |
| Thioaptamers |
Gene silencing activity |
| Antisense |
Lower melting temperature |
| Inhibition of enzyme activity |
Increased siRNA nuclease resistance |
| Distribution: through Glen Research |
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Synthesis of oligos: available through a number of our synthesis partners including Sigma-Custom Products
and the Oligo Factory |
Seeking collaboration partners: AM is actively seeking research partners who are willing to provide data regarding the utility of PS2-modified siRNAs. For our collaborators, AM will provide monomers and advice regarding placement of PS2 modifications. |
Downloads
- DNA Thiophosphoramidite Synthesis Protocol
- DNA Thiophosphoramidite Product Bulletin
- RNA Thiophosphoramidite Product Bulletin
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