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THIOAPTAMER TECHNOLOGY centers around achiral DNA phosphordithioate oligonucleotides. AM Biotechnologies uses a patent pendingbead-based process and patented thiophosphoramidites to simultaneously select the DNA sequence and dithio backbone modifications of a thioaptamer binding agent. In many instances, only one pass through the selection process is required to find a nuclease resistant, achiral binding agent with high specificity and excellent affinity; however, repeating the selection process with a library specifically designed using results from the first selection may prove beneficial. Phosphorodithioate oligos can enhance binding affinity over normal aptamers by a factor of up to 600. |
What are Thioaptamers? Thioaptamers are extremely stable, nucleic acid-based molecules that can be designed with very high specificity and affinity to a wide range of important biomolecular targets. Thioaptamers are unique and proprietary as a result of the chemical substitution of one or two sulfur atoms for the phosphoryl oxygens of an internucleoside phosphate.
Thioaptamer Characteristics
How Thioaptamers are Discovered Selecting phosphorodithioate oligos (thioaptamers) can only be accomplished using AM's proprietary bead-based technology. Read a short paper on thioaptamers. The bead-based discovery process is a unique and novel technology that does not rely on rounds of enrichment of nucleic acids. |
Producing and Using Thioaptamers Thiophosphoramidite reagents are used in conjunction with standard sulfurization reagents to create the phosphorodithioate oligos. Additionally, since thioaptamers are selected while attached to the surface of a bead, it is almost assured that thioaptamers will perform effectively when attached to other surfaces. |
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